The campaign, was started by Brighton resident Adam Mason with the aim of creating a more welcoming public space that is traffic-free and cleaner and safer as a result.
The petition will come to a close on October 23 before being presented to the city council the day after.
In the petition, Adam Mason states that a traffic-free street would facilitate the creation of permanent art installations associated with the community such as the recently suggested Rainbow crossing, which would be similar to the rainbow road created in Iceland.
As well as this, the petition states that closure of the street would also facilitate events such as the Pride Street Party.
Traders on St James’ Street have shown their support for the proposal to pedestrianise the area and the idea was given also a vote of confidence by Lloyd Russell-Moyle, the Labour MP for Brighton Kemptown.
However some Brighton residents have voiced their objections against the proposal to close the road off to vehicles.
Bill Harwood from Hanover said that the plan would leave dozens of roads with dead ends and no turning places which would increase congestion, accidents and create a haven for bad behaviour and rough sleeping.
While Tony Davenport expressed his concerns for the elderly and disabled who need to use the buses that will no longer be able to service pedestrianised areas.
The petition which is currently nearing its close will be presented to the full council on October 24.